QUICK METHOD FOR DETECTING PATHOGENIC BACTERIA BY SERS SPECTROSCOPY

The invention relates to a method for detecting pathogenic bacteria in aqueous media. According to the invention, the method comprises the steps of synthesis of a colloidal suspension of gold colloidal nanoparticles coated with chitosan, concentration in two centrifugation steps for 10 min at 8000 r...

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Bibliographische Detailangaben
Hauptverfasser:	SZOKE-NAGY TIBERIU, TURCU IOAN, JAKAB ENDRE, AŞTILEAN SIMION, POTARA MONICA, JAKAB REKA ILONA
Format:	Patent
Sprache:	eng ; rum
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for detecting pathogenic bacteria in aqueous media. According to the invention, the method comprises the steps of synthesis of a colloidal suspension of gold colloidal nanoparticles coated with chitosan, concentration in two centrifugation steps for 10 min at 8000 rpm, resuspension in distilled water, selection and cultivation of pathogens of the class S. aureus and E. faecalis, placing of a volume of 2 μl of pathogen suspended in distilled water on a glass plate with drying, addition of a volume of 2 μl of colloidal solution, by dripping, with sample drying at room temperature, followed by spectral analysis and image processing, to result in SERS distribution maps wherefrom the biochemical profile of the pathogen is identified, with a detection rate of approximately 30 min.