GROWTH SUPPLEMENT FOR CELL CULTURE MEDIA WITH BENEFICIAL PROPERTIES UPON PROLIFERATION AND VIABILITY OF PRIMARY CULTURES AND CONTINUOUS ANIMAL AND HUMAN CELL LINES

The invention relates to a growth supplement for cell culture media and to a method for culturing cells using said supplement. The claimed supplement is a dipeptide H-Gly-Gly-OH having effect on the proliferation and growth of the cell viability and antioxidant effect. The claimed method consists in...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: BRATOSIN DANIELA, IORD CHEL C T LIN, RUGIN ALEXANDRINA MARIA, SIDOROFF MANUELA ELISABETA, OPRI A ELENA IULIA, DOBRE ANA-MARIA, CIOTEC ANDREEA C T LINA LAVINIA
Format: Patent
Sprache:eng ; rum
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to a growth supplement for cell culture media and to a method for culturing cells using said supplement. The claimed supplement is a dipeptide H-Gly-Gly-OH having effect on the proliferation and growth of the cell viability and antioxidant effect. The claimed method consists in using the supplement in a concentration of 50 μM...10 mM in admixture with a usual culture medium, 5...20% fetal bovine serum and 1% antibiotic mixture for culturing primary cells and continuous cell lines. Invenţia se referă la un supliment de creştere pentru medii de cultură celulare, şi la o metodă de cultivare a celulelor care utilizează acest supliment. Suplimentul conform invenţiei este un dipeptid H-Gly-Gly-OH cu efect de proliferare şi creştere a viabilităţii celulare şi antioxidant. Metoda conform invenţiei constă în utilizarea suplimentului, într-o concentraţie de 50 μM...10 mM în amestec cu un mediu de cultură uzual, 5...20% ser fetal bovin şi 1% amestec antibiotice, pentru cultivarea unor celule primare şi linii celulare continue.