PROCESS FOR OBTAINING AND USING HEN IMMUNOGLOBULINS ()
The invention relates to a process for obtaining hen immunoglobulins IgY. According to the invention, the process comprises the immunization of egg-laying hens with a previously prepared antigen consisting of a mixture of bacterial strains resistant to antibiotics, the partial purification of IgY ex...
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Format: | Patent |
Sprache: | eng ; rum |
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Zusammenfassung: | The invention relates to a process for obtaining hen immunoglobulins IgY. According to the invention, the process comprises the immunization of egg-laying hens with a previously prepared antigen consisting of a mixture of bacterial strains resistant to antibiotics, the partial purification of IgY extracted from the yolk, at pH = 5, by quick freezing for 60 min, at a temperature of -40°C and quick defrosting, at a temperature of +20°C, followed by the concentration of the resulting IgY by tangential filtration.
Invenţia se referă la un procedeu de obţinere a imunoglobulinelor de găină IgY. Procedeul conform invenţiei constă în imunizarea găinilor ouătoare cu un antigen preparat în prealabil, constând dintr-un amestec de tulpini bacteriene rezistente la antibiotice, purificarea parţială a IgY extras din galbenuş, laH = 5, prin congelare rapidă, timp de 60 min, la o temperatură de -40°C, şi decongelare rapidă la +20°C, urmată de concentrarea IgY obţinute prin filtrare tangenţială. |
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