PROCESS FOR ENHANCING THE ANTIMICROBIAL EFFECT OF GENTAMICIN

The invention relates to a process for enhancing the antimicrobial activity of gentamicin. According to the invention, the process consists in selectively removing the gentamicin C, as the component having the lowest biological activity, from the solution resulting from biosynthesis, by the reactive...

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Bibliographische Detailangaben
Hauptverfasser: CA CAVAL DAN, GALACTION ANCA IRINA, BLAGA ALEXANDRA CRISTINA
Format: Patent
Sprache:eng ; rum
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Zusammenfassung:The invention relates to a process for enhancing the antimicrobial activity of gentamicin. According to the invention, the process consists in selectively removing the gentamicin C, as the component having the lowest biological activity, from the solution resulting from biosynthesis, by the reactive extraction of a gentamicin-containing aqueous solution, in a ratio of 1:1, balanced to a pH value = 8 with di-chloro-methane containing 1...6% di(2-ethylhexyl)phosphoric acid, at a temperature of 25°C while stirring vigorously for 1 min, followed by separation and re-extraction of the gentamicins Cand Cof the organic phase, in a ratio of 1:1, with an aqueous solution of sulphuric acid in a concentration of 0.2...1.1%, at a temperature of 25°C, while stirring vigorously for 1 min. Invenţia se referă la un procedeu de mărire a activităţii antimicrobiene a gentamicinei. Procedeul conform invenţiei constă din îndepărtarea selectivă a gentamicinei C, componentă cu cea mai scăzută activitate biologică, din soluţia rezultată în urma biosintezei, prin extracţia reactivă în raport 1:1 a soluţiei apoase conţinând gentamicina, ajustată la o valoare pH = 8, cu diclormetan conţinând 1-6 % acid di(2-etilhexil)fosforic, la 25°C, sub agitare intensă timp de 1 min, urmată de separarea şi reextracţia gentamicinelor Cşi Cdin faza organică, în raport 1:1, cu soluţie apoasă 0,2...1,1% de acid sulfuric, la 25°C, sub agitate intensă timp de 1 min.