VITROCERAMIC COMPOSITE AND PROCESS FOR THE IMMOBILIZATION OF L-ASPARTIC ACID AND L-ASPARAGINE THEREON

The invention relates to a vitroceramic composite for the fixation of L-aspartic acid and L-asparagine and to a process for the immobilization of L-aspartic acid and L-asparagine on the above-mentioned composite, in order to prepare affinitive effectors for the isolation of L-asparagine (E.C. 3.1.1....

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Hauptverfasser:	TCACENCO LUMINI A, LUPESCU IRINA, TEODOR EUGENIA-DUMITRA, STAN CRISTIANA
Format:	Patent
Sprache:	eng ; rum
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL COMPOSITION OF GLASSES, GLAZES, OR VITREOUSENAMELS CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY GLASS JOINING GLASS TO GLASS OR OTHER MATERIALS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MINERAL OR SLAG WOOL MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS,MINERALS OR SLAGS SURFACE TREATMENT OF GLASS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a vitroceramic composite for the fixation of L-aspartic acid and L-asparagine and to a process for the immobilization of L-aspartic acid and L-asparagine on the above-mentioned composite, in order to prepare affinitive effectors for the isolation of L-asparagine (E.C. 3.1.1.5.) by affinity chromatography. According to the invention, the vitroceramic composition comprises 43.2 ... 49.4% SiO2, 13.2 ... 17.8% MgO, 17.80 ... 32.30% Al2O3, 0 ... 4.40 % CaO, 0 ... 0.20% Wa2O, 0 ... 0.24 % K2O, 2.00 ... 11.00% TiO2, 0 ... 10.28% CeO2. The immobilization of L-aspartic acid and L-asparagine on the vitroceramic composition is achieved by progressive adsorbtion up to saturation of aminoacids on carrier, while stirring at 200 rpm for 30 min. Invenţia se referă la un compozit vitroceramic,pentru fixarea acidului L-aspartic şi a L-asparaginei, şi la un procedeu de imobilizare a acidului L-aspartic şi a L-asparaginei pe compozitul menţionat, în scopul obţinerii de efectori afini pentru izolarea L-asparaginazei (E. C. 3. 1. 1. 5),prin cromatografie de afinitate. Compozitul vitroceramic conform invenţiei este constituit din 43,2...49,4% SiO2, 13,2...17,8% MgO, 17,80...32,30% Al2O3, 0...4,40% CaO, 0...0,20% Wa2O, 0...0,24% K2O, 2,00...11,00% TiO2, 0...10,28% CeO2. Imobilizarea acidului L-aspartic şi a L-asparaginei pe compozitul vitroceramic se realizează prin adsorbţiaprogresivă, până la saturare, a aminoacizilor pe suport, în condiţii de agitare la 200 rpm, la20°C, timp de 30 min.