NUCLEOSIDASE, NUCLEOTIDES AND OLIGONUCLEOTIDE WHICH ARE COUPLED WITH FLUORESCENT COLORING MATTERS AND PREPARATION PROCESS THEREFOR

The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oli...

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Bibliographische Detailangaben
Hauptverfasser:	RENATE KONRAD, JOACHIM ENGELS, MATHIAS HERRLEIN, MATTHIAS MAG
Format:	Patent
Sprache:	eng ; rum
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of items in the presence of a template strand or of oligonucleotides, and for the detection of genetic material. They offer the advantage that the fluorescent label is no longer attached to the 5' end of the oligonucleotide or to the nucleobase and thus does not have to be introduced during the chemical synthesis as in labelling techniques hitherto disclosed, while the known and conventional methods have the disadvantage that only some polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases falls and, in addition, a large substrate excess is necessary.