ISOLATION METHOD OF HUMAN Ó1- ANTITRYPSIN
Urine or human serum contg. alpha-1-antitrypsin (Li-AT) is pptd. with an equivalent quantity of monospecific antiLiAT, the immune complex is sepd. by centrifugation and dried with 0.05% SDS, centrifuged, pptd. using min. 0.05M tris HCl at pH8 contg. 0.8% SDS and gel filtered by known methods e.g. by...
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Zusammenfassung: | Urine or human serum contg. alpha-1-antitrypsin (Li-AT) is pptd. with an equivalent quantity of monospecific antiLiAT, the immune complex is sepd. by centrifugation and dried with 0.05% SDS, centrifuged, pptd. using min. 0.05M tris HCl at pH8 contg. 0.8% SDS and gel filtered by known methods e.g. by Bio-gel p-100 fine column in buffer. |
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