PRODUCTION METHOD OF IMMUNO-GLOBULIN A1(IgA1)

PRODUCTION METHOD OF IMMUNO-GLOBULIN A1(IgA1)

The process comprises defibrination with CaCl2 of plasma very high in IgA, and very low in IgG, for treatment with 25 g of (NH4)2SO4 per 100 ml at 4 deg.C. The centrifuged ppte is washed two hours with soln. of (NH4)2SO4 (25%, 25g per 100 ml of water), and buffered with Na3PO4/H3PO4, 0.03 M, pH = 5,...

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Bibliographische Detailangaben
Hauptverfasser: IONITA AUREL, IONITA CONSTANTINA, IONITA EMIL
Format: Patent
Sprache:eng
Schlagworte:
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Zusammenfassung:The process comprises defibrination with CaCl2 of plasma very high in IgA, and very low in IgG, for treatment with 25 g of (NH4)2SO4 per 100 ml at 4 deg.C. The centrifuged ppte is washed two hours with soln. of (NH4)2SO4 (25%, 25g per 100 ml of water), and buffered with Na3PO4/H3PO4, 0.03 M, pH = 5, containing 0.3 M of NaCl and 0.02 per cent of NaN3. Chromatography, and elution, with buffer K2HPO4, 0.02 M, pH = 8, and further chromatography, is followed by elution with 0.1 M NaCl in K2HPO4, 0.02 M, pH = 8.