PROCESSO PARA A PREPARACAO DE PURIFICACAO DE UMA PROTEINA A PARTIR DE UMA PLANTA COM UM EFEITO INIBIDOR NUM PATOGENIO OU PESTE E DE COMPOSICOES ANTI-FUNGOS QUE AS CONTEM

New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fractionation techniques on leaf-extracts of plants which were...

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Hauptverfasser:	CHARLES PETER WOLOSHUK, ELISABETH JOSINE SOPHIE MEULENHOFF, LEO SJOERD MELCHERS, PETRUS JOSEPHUS MARIA VAN DEN ELZEN, MARIANNE BEATRIX SELA-BUURLAGE, BERNARDUS JOHANNES CLEMENS CORNELISSEN
Format:	Patent
Sprache:	por
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fractionation techniques on leaf-extracts of plants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the said method, recombinant nucleotide sequences encoding the said proteins, as well as plants with reduced susceptibility to fungi due to production and desired targetting of the said proteins in the plant.