PROCESS FOR ISOLATE AND PURIFY CONCERNING A TISSUE PLASMINOGEN ACTIVATOR

PROCESS FOR ISOLATE AND PURIFY CONCERNING A TISSUE PLASMINOGEN ACTIVATOR

A process for isolating tPA which comprises contacting a cell culture medium with a solid support onto which the tPA becomes adsorbed, washing the support with water buffered to < pH 5 and then eluting the tPA with an aqueous solution of an alkali or alkaline earth metal salt or a chaotrope buffe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ROBERT DAVID SITRIN, SAMUEL GREGG FRANKLIN, KYUNG OH JOHANSON, JAMES EDWARD STRICKLER, PATRICK JOSEPH MCDEVITT, PETER ARLETTI DEPHILLIPS
Format: Patent
Sprache:por
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A process for isolating tPA which comprises contacting a cell culture medium with a solid support onto which the tPA becomes adsorbed, washing the support with water buffered to < pH 5 and then eluting the tPA with an aqueous solution of an alkali or alkaline earth metal salt or a chaotrope buffered to < pH 5.