SELECTIVE AMPLIFICATION OF DESIRED NUCLEIC ACID REGIONS IN A TARGET SEQUENCE

A method and kit for selective amplification of desired amplicons in a two-stage polymerase chain reaction is provided. The method and kit uses target primers comprising mismatch control sequences; wherein any pair of primers that flank a desired amplicon sequence have non-matching mismatch control...

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Bibliographische Detailangaben
Hauptverfasser:	STEFFEN HEIM, ULF KLANGBY, ANDERS HEDRUM
Format:	Patent
Sprache:	eng ; por
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method and kit for selective amplification of desired amplicons in a two-stage polymerase chain reaction is provided. The method and kit uses target primers comprising mismatch control sequences; wherein any pair of primers that flank a desired amplicon sequence have non-matching mismatch control sequences. and any pair of primers that flank an undesired amplicon sequence have matching mismatch control sequences.