PROCESS FOR THE PREPARATION OF INSULIN CONJUGATES

PROCESS FOR THE PREPARATION OF INSULIN CONJUGATES

The invention claims a process for making an insulin-oligomer conjugate IN-105. IN-105 precursor having formula G-A-V-R-[B-Chain]-R-D-A-D-D-R-[A-Chain] is cloned and expressed in Pichia. The biosynthetic precursor is then conjugated with an activated oligomer. The IN-105 precursor-oligomer conjugate...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: PARTHA HAZRA, ANUJ GOEL, GOVINDASAMY MANIKAM, HARISH IYER, GAUTAM KRISHNAN, SHRIKUMAR SURYANARAYAN, ANAND KHEDKAR, NITA ROY, GOLDY SACHDEV, NITESH DAVE, H. S. MANJUNATH, MAYANK GARG
Format: Patent
Sprache:eng ; por
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:The invention claims a process for making an insulin-oligomer conjugate IN-105. IN-105 precursor having formula G-A-V-R-[B-Chain]-R-D-A-D-D-R-[A-Chain] is cloned and expressed in Pichia. The biosynthetic precursor is then conjugated with an activated oligomer. The IN-105 precursor-oligomer conjugate is then treated with protease and purified to afford active insulin-oligomer conjugate of formula insulin-OC-CH2-CH2-(OCH2CH2)3-OCH3.