SEQUENTIAL PROTEIN ISOLATION AND PURIFICATION SCHEMES BY AFFINITY CHROMATOGRAPHY

The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligan...

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Bibliographische Detailangaben
Hauptverfasser: BALDEV BAINES, JOHN CURLING, CHRISTOPHER BRYANT, DAVID JOHN HAMMOND, TIMOTHY KEITH HAYES, DWUN-HOU CHEN, STEVEN JAMES BURTON
Format: Patent
Sprache:eng ; por
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Beschreibung
Zusammenfassung:The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligands or ligand support complexes were contacted sequentially in a predetermined order with the biological sample to allow each ligand or ligand-support complex to sequentially bind a protein from the biological sample.