METHOD FOR EXTRACTING GLUTEN CONTAINED IN HEAT-PROCESSED AND NON-HEAT-PROCESSED FOODSTUFFS, COMPATIBLE WITH AN ENZYME-LINKED IMMUNOSORBENT ASSAY, COMPOSITION AND KITS COMPRISING SAID COMPOSITION

METHOD FOR EXTRACTING GLUTEN CONTAINED IN HEAT-PROCESSED AND NON-HEAT-PROCESSED FOODSTUFFS, COMPATIBLE WITH AN ENZYME-LINKED IMMUNOSORBENT ASSAY, COMPOSITION AND KITS COMPRISING SAID COMPOSITION

The invention relates to a method for extracting the gluten contained in a sample consisting in using an ethanol aqueous solution in the presence of a composition containing a disulphur group reducer and a decoupling agent, in a buffer having a pH of between 7 and 8. The inventive method can be used...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ENRIQUE MENDEZ CORMAN, ELENA LOPEZ VILLAR, MERCEDES LLORENTE GOMEZ
Format: Patent
Sprache:eng ; por
Schlagworte:
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:The invention relates to a method for extracting the gluten contained in a sample consisting in using an ethanol aqueous solution in the presence of a composition containing a disulphur group reducer and a decoupling agent, in a buffer having a pH of between 7 and 8. The inventive method can be used to extract quantitatively the gluten contained in a heat-processed or non-heat processed food sample before the quantification of the gluten by ELISA. Said method is suitable for food analyses, in particular, for foodstuffs intended for celiac sufferers.