PLATELET LYSATE-BASED MICROPARTICLES, METHODS AND USES THEREOF

THE PRESENT DISCLOSURE RELATES TO A PROCESS TO ASSEMBLE PROTEINS DERIVED FROM PLATELET LYSATES IN BIOACTIVE MICROPARTICLES, WITH INCREASED SURFACE ORGANIZATION. THE PRESENT INVENTION FURTHER RELATES TO PROTEIN-BASED BIOMATERIALS APPLICABLE TO BIOMEDICAL AND BIOTECHNOLOGY FIELDS, MORE PRECISELY IN TISSUE ENGINEERING STRATEGIES, DISEASE MODELING, AND OTHER BIOMEDICAL APPLICATIONS. NAMELY, A METHOD FOR OBTAINING A PROTEIN MICROPARTICLE FROM A PLATELET LYSATE COMPRISING THE FOLLOWING STEPS: LYOPHILIZING THE PLATELET LYSATE; DISSOLVING THE LYOPHILIZED PLATELET LYSATE IN PHOSPHATE BUFFER SALINE TO OBTAIN A PLATELET LYSATE SOLUTION; ADDING A REDUCING AGENT TO THE PLATELET LYSATE SOLUTION TO OBTAIN A REDUCED PLATELET LYSATE SOLUTION; ADDING AN OXIDIZING AGENT TO THE REDUCED PLATELET LYSATE SOLUTION TO FORM THE MICROPARTICLES BY PRECIPITATION. The present disclosure relates to a process to assemble proteins derived from platelet lysates in bioactive microparticles, with increased surface organization. The present invention further relates to protein-based biomaterials applicable to biomedical and biotechnology fields, more precisely in tissue engineering strategies, disease modeling, and other biomedical applications. Namely, a method for obtaining a protein microparticle from a platelet lysate comprising the following steps: lyophilizing the platelet lysate; dissolving the lyophilized platelet lysate in phosphate buffer saline to obtain a platelet lysate solution; adding a reducing agent to the platelet lysate solution to obtain a reduced platelet lysate solution; adding an oxidizing agent to the reduced platelet lysate solution to form the microparticles by precipitation.