PURIFIED AND ISOLATED DNA SEQUENCE ENCODING A POLYPEPTIDE OF XYLANASE ACTIVITY AND METHOD OF OBTAINING SUCH SEQUENCE, DNA EXPRESSION STRUCTURE, METHOD OF OBTAINING SAME, TRANSFORMED HOST OF MICROBIAL ORIGIN AND METHOD OF OBTAINING SAME, METHOD OF XYLANASE ACTIVITY POLYPEPTIDE HYPEREXPRESSION, POLYPEPTIDE OF XSYLANASE ACTIVITY AND APPLICATION OF SUCH POLYPEPTIDE, ANIMAL FEED AND METHOD OF OBTAINING SAME, DOUGH FOR BAKING BREAD, METHOD OF DEGRADATING PRODUCTS CONTAINING XYLANES

Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH rang...

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Bibliographische Detailangaben
Hauptverfasser:	HARDER ABRAHAM, VAN DEN BROECK HENRIETTE C, OOYEN ALBERT J. J, DE GRAAFF LEENDERT H, VISSER JOCOB, HILLE JAN D. R
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS THEREFOR BAKERY PRODUCTS BAKING BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA EDIBLE DOUGHS ENZYMOLOGY FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PAPER-MAKING PRESERVATION THEREOF PROCESSES USING MICROORGANISMS PRODUCTION OF CELLULOSE PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REGENERATION OF PULPING LIQUORS SPIRITS TEXTILES THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH range than do xylanases of bacterial origin. The present invention provides for the high level production of fungal xylanases which are may be used in a variety of industrial applications requiring xylanase activity at a low pH.