7-beta-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid

The invention relates to novel 7β-hydroxysteroid dehydrogenase mutants, to sequences encoding said enzyme mutants, to methods for the preparation of the enzyme mutants and to their use in enzymatic reactions of cholic acid compounds, and especially in the production of ursodeoxycholic acid (UDCA). T...

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Bibliographische Detailangaben
Hauptverfasser:	Hummel, Werner, Bakonyi, Daniel
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to novel 7β-hydroxysteroid dehydrogenase mutants, to sequences encoding said enzyme mutants, to methods for the preparation of the enzyme mutants and to their use in enzymatic reactions of cholic acid compounds, and especially in the production of ursodeoxycholic acid (UDCA). The invention also relates to novel processes for the synthesis of UDCA using the enzyme mutants; and to the preparation of UDCA using recombinant microorganisms that have been subjected to multiple modifications. Said mutant has increased specific activity (Vmax [U/mg]) for dehydrocholic acid (DHCA) in the enzymatic reduction of DHCA, as well as for NAD(P)H in the enzymatic reduction of DHCA with NAD(P)H as cofactor.