Fluidic peristaltic layer pump

A microfluidic device is provided for managing fluid flow in disposable assay devices, which provides constant flow even at very low flow rates. The device includes a rigid body having a first curved slot disposed therein and a rigid substrate having a top surface attached to the rigid body. The rigid substrate including a first inlet port and a first outlet port disposed in the top surface and positioned in alignment with a first end and a second end of the first curved slot. The device further includes a first elastic member disposed within the first curved slot and having a first surface and a second surface, wherein the second surface comprises a groove defining a first channel with the rigid substrate. Pumps utilizing the microfluidic device, as well as methods for manufacture and performing a microfluidic process are also provided.