Methods and test kits for determining male fertility status

Methods and test kits for determining male fertility status

This disclosure provides a method for determining male fertility status. The method comprises determining more than one GM1 localization patterns following induced sperm capacitation selected from the group consisting of: apical acrosome (AA), acrosomal plasma membrane (APM), Lined-Cell, post acroso...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Moody, Melissa, Travis, Alexander, Ostermeier, G, Cardona, Cristina, Simpson, Alana
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:This disclosure provides a method for determining male fertility status. The method comprises determining more than one GM1 localization patterns following induced sperm capacitation selected from the group consisting of: apical acrosome (AA), acrosomal plasma membrane (APM), Lined-Cell, post acrosomal plasma membrane (PAPM), apical acrosome/post acrosome (AA/PA), intermediate (INTER), equatorial segment (ES) and diffuse (DIFF) GM1 localization patterns; assigning the AA and APM patterns to a capacitated state, identifying the percentage of various patterns, particularly the ratio of [(AA+APM)/total number of GM1 localization patterns] and determining if the percentage of certain GM1 localization patterns in response to induced capacitation is altered. In a particular embodiment, the total number of GM1 localization patterns comprises AA, APM, Lined-Cell, PAMP, AA/PA, INTER, ES and DIFF localization patterns. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.