Methods for transduction and cell processing

Provided are methods, systems, and kits for cell processing, e.g., for therapeutic use, such as for adoptive cell therapy. The claimed method is a transduction method, in which cells and virus are incubated under conditions that result in transduction of the cells with a viral vector. The incubation is carried out in a variable volume internal cavity of a generally rigid centrifugal chamber, such as a cylindrical chamber made of hard plastic. The volume is varied by a movable member. The method includes other processing steps, including those carried out in such a chamber, including washing, selection, isolation, culture, and formulation. In particular, the disclosure relates to method providing advantages over available processing methods, such as available methods for large-scale processing. Such advantages include, for example, reduced cost, streamlining, increased efficacy, increased safety, and increased reproducibility among different subjects and conditions.