Temperature shift for high yield expression of polypeptides in yeast and other transformed cells

Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. The transformed cells used are a methylotrophic yeas...

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Bibliographische Detailangaben
Hauptverfasser:	Mcneill, Patricia Dianne, Lesnicki, Gary, Young, Mark, Hartner, Franz
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. The transformed cells used are a methylotrophic yeast of the genus Pichia, more specifically Pichia pastoris. The method further involves culturing the cells at two different temperatures, the first being between 27.5-28.5 degrees celsius and the second being between 30-31 degrees celsius. Additionally, the promoter used to control expression of the desired antibody is not temperature inducible.