Expression of chimeric ksac protein and method of producing soluble proteins by high pressure

Methods for producing a soluble protein expressed in prokaryotes or eukaryotes comprising the steps of (i) preparing inclusion bodies in a buffer comprising 0M to about 2M urea to form an inclusion body suspension; (ii) subjecting the inclusion body suspension to a target high pressure consisting of...

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Bibliographische Detailangaben
Hauptverfasser:	LUX FABIEN, FISCHER LAURENT BERNARD, CARBOULEC NICOLAS PIERRE YVES
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for producing a soluble protein expressed in prokaryotes or eukaryotes comprising the steps of (i) preparing inclusion bodies in a buffer comprising 0M to about 2M urea to form an inclusion body suspension; (ii) subjecting the inclusion body suspension to a target high pressure consisting of a stepwise increase of pressure over a period of time; and (iii) maintaining the high pressure applied to the inclusion bodies for a period of time.