Systems and methods for ex-vivo organ care

Disclosed is a heart preservation and transport system including a single use module for improved organ transplantation. The organ chamber assembly for containing an organ for preservation comprises a housing having a bottom and walls sized for containing the organ, wherein the organ is a heart. An intermediate lid is use for covering an opening to the housing for substantially enclosing the organ within the housing, the intermediate lid including a frame sized to substantially cover an outer rim of the opening. A flexible membrane is attached to and suspended within the frame, the flexible membrane being configured to allow an operator to manipulate an organ contained within the chamber while still maintaining sterility of the system and the organ. The intermediate lid being coupled to the housing an intermediate lid hinge such that the intermediate lid is independently movable from the housing; and an outer lid independently operable from the intermediate lid for covering, at least partially, the intermediate lid, the outer lid being coupled to at least one of the intermediate lid and the housing by an outer lid hinge.