Method for improved transformation using agrobacterium

Disclosed is a method for plant cell transformation comprising exposing immature embryo plant cells to Agrobacterium cells in a liquid medium containing a non-ionic trisiloxane surfactant, the surfactant having a concentration of 0.001 weight percentage to 0.08 weight percentage in the liquid medium...

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Bibliographische Detailangaben
1. Verfasser: Miller Paul David
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Disclosed is a method for plant cell transformation comprising exposing immature embryo plant cells to Agrobacterium cells in a liquid medium containing a non-ionic trisiloxane surfactant, the surfactant having a concentration of 0.001 weight percentage to 0.08 weight percentage in the liquid medium, and wherein the non-ionic trisiloxane surfactant is not a trisiloxane alkoxylate.