Keto-isovalerate decarboxylase enzymes and methods of use thereof

Discloses a method of producing isobutanol comprising: a. providing a recombinant host cell comprising an isobutanol biosynthetic comprising a polypeptide having at least 80% identity to SEQ ID NO:52 or 61; and b. contacting the recombinant host cell with a carbon substrate under conditions whereby...

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Bibliographische Detailangaben
Hauptverfasser:	PAYNE MARK S, MCELVAIN JESSICA, HE HONGXIAN, PAUL BRIAN JAMES, O'KEEFE DANIEL P, ROTHMAN STEVEN CARY
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE VINEGAR WINE
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Beschreibung
Zusammenfassung:	Discloses a method of producing isobutanol comprising: a. providing a recombinant host cell comprising an isobutanol biosynthetic comprising a polypeptide having at least 80% identity to SEQ ID NO:52 or 61; and b. contacting the recombinant host cell with a carbon substrate under conditions whereby isobutanol is produced; wherein the method is not carried out in a human cell; wherein the sequences are as defined in the complete specification.