Methods for improving viability and productivity in cell culture

Methods for improving viability and productivity in cell culture

Patent No. 602853 Disclosed is a method of producing a secreted protein in a Chinese Hamster Ovary (CHO) fed batch cell culture, comprising culturing a CHO cell line over-expressing MDM2 and E1B19K and one or more genes encoding the secreted protein, wherein the titer of the produced secreted protei...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: SAUERWALD MCLAIN TINA M, DORAI HAIMANTI, LY CELIA
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Patent No. 602853 Disclosed is a method of producing a secreted protein in a Chinese Hamster Ovary (CHO) fed batch cell culture, comprising culturing a CHO cell line over-expressing MDM2 and E1B19K and one or more genes encoding the secreted protein, wherein the titer of the produced secreted protein is at least 600 mg/L at day 23 of the fed batch culture.