METHOD AND DEVICE FOR CELL CULTURE IN OPEN CONTINUOUS MODE

Method for continuous cell culture allowing the selection of cell variants that are static or proliferating in suspension, which comprises the following stages: a) a liquid culture medium contained in a first culture vessel kept open in a closed container is seeded using one or more live cells, b) said cells, in said culture medium, are brought to a specific growth stage, corresponding to a given cell density or to a physicochemical parameter measured in the culture medium, c) the cell density in the culture medium, or the value of said physicochemical parameter, reached in stage b), is kept substantially constant by supplying fresh culture medium or a diluent in said culture vessel, d) a portion of the culture medium of the culture obtained in c) containing the cells in suspension is removed by pipetting so as to maintain the volume of the culture, e) a fraction of the culture medium obtained in d) is transferred into a second culture vessel, f) said first culture vessel with the remaining culture fraction that it contains is withdrawn; g) after several generations of culture in the second vessel, the cells proliferating in suspension and/or the static cell variants are selected.