Process for obtaining growth factor (TGF-beta and IGF-1), lactoperoxidase and immunoglobulins preparations from milk products having low mutual cross-contamination

Disclosed is a process for extracting fractions containing the growth factors TGF-B and IGF-1 from a milk product, comprising the steps of: recovering a basic fraction of the milk product by means of cationic exchange chromatography; contacting a solution containing the obtained fraction with a hydr...

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Bibliographische Detailangaben
Hauptverfasser:	GALAMA, CATHARINA MARIA, HENDRIKS, ANDOR WILHEIM JOSEPH, KIVITS, MARINUS GERARDUS CORNELIS
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PROTEIN COMPOSITIONS FOR FOODSTUFFS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE WORKING-UP PROTEINS FOR FOODSTUFFS
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Beschreibung
Zusammenfassung:	Disclosed is a process for extracting fractions containing the growth factors TGF-B and IGF-1 from a milk product, comprising the steps of: recovering a basic fraction of the milk product by means of cationic exchange chromatography; contacting a solution containing the obtained fraction with a hydrophobic interaction chromatography resin comprising a carrier and a ligand attached to the carrier; wherein the ligand of the hydrophobic interaction chromatography resin is selected from a n-butyl group, a t-butyl group, and a phenyl group; eluting the hydrophobic interaction chromatography resin with an eluent to obtain a fraction containing growth factor compounds; and wherein the eluent contains substantially no alcohol when a phenyl group is used as a ligand.