Methods of producing and screening mutants of a Mycobacterium species of the M. tuberculosis complex for attenuation
Methods of screening for attenuated strains of Mycobacterium tuberculosis complex, which comprise at least one inactive gene, are described. The screening comprises steps of isolating a Mycobacterium species to be screened, growing the isolated mutants for 5-10 weeks in a complete medium, transferri...
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| creator | COLLINS, DESMOND MICHAEL WARDS, BARRY JOHN KAWAKAMI, RIKU PAMELA WILSON, THERESA MARY CAMPBELL, SHALOME ANITTA |
| description | Methods of screening for attenuated strains of Mycobacterium tuberculosis complex, which comprise at least one inactive gene, are described. The screening comprises steps of isolating a Mycobacterium species to be screened, growing the isolated mutants for 5-10 weeks in a complete medium, transferring the mutants to minimal media and selecting the mutants based on their inability to grow in minimal media. The figure shows a Southern blot hybridization after EcoR1 digestion and probing with neo of eight recombinants that were unable to grow in a minimal medium (lanes 1-5 and 7-9) and one recombinant that failed the screen and grew in the minimal medium (lane 6). (62) Divided out of 519395 |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Methods of producing and screening mutants of a Mycobacterium species of the M. tuberculosis complex for attenuation |
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