Methods of producing and screening mutants of a Mycobacterium species of the M. tuberculosis complex for attenuation

Methods of producing and screening mutants of a Mycobacterium species of the M. tuberculosis complex for attenuation

Methods of screening for attenuated strains of Mycobacterium tuberculosis complex, which comprise at least one inactive gene, are described. The screening comprises steps of isolating a Mycobacterium species to be screened, growing the isolated mutants for 5-10 weeks in a complete medium, transferri...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: COLLINS, DESMOND MICHAEL, WARDS, BARRY JOHN, KAWAKAMI, RIKU PAMELA, WILSON, THERESA MARY, CAMPBELL, SHALOME ANITTA
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Methods of screening for attenuated strains of Mycobacterium tuberculosis complex, which comprise at least one inactive gene, are described. The screening comprises steps of isolating a Mycobacterium species to be screened, growing the isolated mutants for 5-10 weeks in a complete medium, transferring the mutants to minimal media and selecting the mutants based on their inability to grow in minimal media. The figure shows a Southern blot hybridization after EcoR1 digestion and probing with neo of eight recombinants that were unable to grow in a minimal medium (lanes 1-5 and 7-9) and one recombinant that failed the screen and grew in the minimal medium (lane 6). (62) Divided out of 519395