Isolation TGF-beta and IGF-1 as pure fractions and high yield recovery of native lactoperoxidase from milk products

Isolation TGF-beta and IGF-1 as pure fractions and high yield recovery of native lactoperoxidase from milk products

Discribed is a process for extracting and transforming growth factor beta (TGF-beta ) and insulin-like growth factor 1 (IGF-1) from a milk product. The process comprises steps of: a) recovering a basic fraction from the milk product by means of cationic exchange chromatography; b) passing the fracti...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MALLEE, LEONARD FRANCISCUS, HENDRICKS, ANDORWILHELM JOSEPH, KIVITS, MARINUS GERARDUS CORNELIS
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
FOODS OR FOODSTUFFS
HUMAN NECESSITIES
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
PROTEIN COMPOSITIONS FOR FOODSTUFFS
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
WORKING-UP PROTEINS FOR FOODSTUFFS
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Beschreibung
Zusammenfassung:Discribed is a process for extracting and transforming growth factor beta (TGF-beta ) and insulin-like growth factor 1 (IGF-1) from a milk product. The process comprises steps of: a) recovering a basic fraction from the milk product by means of cationic exchange chromatography; b) passing the fraction obtained in step a) over a hydroxyapatite column; c) eluting the hydroxyapatite column with appropriate eluents in such a way as to obtain two separate fractions, these fractions being: i) a fraction comprising IGF-1 in the substantial absence of TGF-beta ; ii) a fraction comprising TGF-beta in the substantial absence of IGF-1. This process can comprise a further step d) in which a lactoperoxidase fraction can be obtained. Also described are the products obtained with this process.