Rapid method for measuring complex carbohydrates in mammalian tissue

Described is a rapid method of measuring complex carbohydrates in non-human mammalian tissue. The method comprises the steps of: a) extracting a sample of tissue to be tested; b) forming a homogenous slurry of the sample with an aqueous solution; c) adding sufficient hydrolysing enzyme for ensuring...

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Bibliographische Detailangaben
Hauptverfasser: HART, ALAN LEEDHAM, YOUNG, OWEN ARCHIBALD, WEST, JOHN
Format: Patent
Sprache:eng
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Zusammenfassung:Described is a rapid method of measuring complex carbohydrates in non-human mammalian tissue. The method comprises the steps of: a) extracting a sample of tissue to be tested; b) forming a homogenous slurry of the sample with an aqueous solution; c) adding sufficient hydrolysing enzyme for ensuring complete hydrolysis of glycogen in the slurry; and d) measuring the concentration of glucose in the slurry. The method can be conducted post-mortem to assay the concentration at the time of slaughter. Also described is the measurement of lactate concentrations. The method can be conducted in thirty minutes or less.