Methods for manipulating the avian genome

A method for introducing a nucleic acid molecule into the genome of an avian species, comprising contacting in vivo a blastodermal cell in a laid fertilized egg with an isolated nucleic acid molecule, wherein (a) said nucleic acid molecule is not associated with a viral coat protein, (b) wherein sai...

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Bibliographische Detailangaben
Hauptverfasser:	DITULLIO, PAUL A, EBERT, KARL M
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for introducing a nucleic acid molecule into the genome of an avian species, comprising contacting in vivo a blastodermal cell in a laid fertilized egg with an isolated nucleic acid molecule, wherein (a) said nucleic acid molecule is not associated with a viral coat protein, (b) wherein said nucleic acid molecule is introduced directly into the germinal disc of said egg in a volume of greater than 1 microlitre and less than 0.5 millilitres, and (c) said nucleic acid molecule is integrated into a host chromosome of said blastodermal cell, thereby introducing said nucleic acid molecule into the genome of said avian species.