DNA ASSAY USING IMMOBILISED PROBES HYBRIDISABLE WITH THE SEQUENCE TO BE DETECTED

A method for detecting one or more microorganisms or polynucleotide sequences from eukaryotic sources in a nucleic acid-contg. test sample comprises (a) preparing a test sample comprising labelling the nucleic acids in the test sample, (b) preparing one or more probes by immobilising an oligonucleot...

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Bibliographische Detailangaben
Hauptverfasser:	HUGUENEL, E D, NANIBHUSHAN DATTAGUPTA, RAE, P M M, RABIN, D U
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for detecting one or more microorganisms or polynucleotide sequences from eukaryotic sources in a nucleic acid-contg. test sample comprises (a) preparing a test sample comprising labelling the nucleic acids in the test sample, (b) preparing one or more probes by immobilising an oligonucleotide or a single-stranded nucleic acid of one of more known microorganisms or sequences from eucaryotic sources, (c) contacting under hybridisation conditions, the labelled single-stranded sample nucleic acid and the immobilised oligonucleotide or single-stranded nucleic acid and (d) assaying for the hybridised nucleic acids by detecting the label.