DNA CODING FOR FUNGAL GLUCOAMYLASE

The invention relates to a process for secreting a proteinaceous material extracellularly which comprises growing a host organism which is transformed by a DNA expression vector comprising a promoter fragment which functions in the host organisms, a signal sequence having substantially the following...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	INNIS, M.A, GELFAND, D.H, NUNBERG, J.H, MEADE, J.H, FLATGAARD, J.E
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a process for secreting a proteinaceous material extracellularly which comprises growing a host organism which is transformed by a DNA expression vector comprising a promoter fragment which functions in the host organisms, a signal sequence having substantially the following amino acid sequence: MET SER PHE ARG SER LEU LEU ALA LEU SER GLY LEU VAL CYS THR GLY LEU ALA ASN VAL ILE SER LYS ARG, and a DNA segment which codes for the proteinaceous material, where said proteinaceous material is characterized as a cell protein which is synthesized in its normal host organism with a hydrophobic signal sequence which is normally cleaved during secretion from its normal host organism. The invention further relates to the above signal sequence.