ISOLATION OF PLASMIDS FROM METHYLOMONAS CLARA;RECOMBINANT DNA METHOD,WITH OBLIGATE METHYLOTROPHIC BACTERIA AS RECIPIENTS;HYBRID PLASMIDS

1. Claims for the Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL, SE A hybrid plasmid with a plasmid replicon inherent to an obligate methylotrophic bacterium. 1. Claims for the Contracting State AT A process for the preparation of obligate methylotrophic bacteria, which express contained foreign DNA, which comprises a) isolating a plasmid from an obligate methylotrophic bacterium, b) preparing from this and from a plasmid with selection markers a hybrid plasmid with a replicon inherent to the obligate methylotrophic bacterium, c) introducing this hybrid plasmid by transformation into a host organism and amplifying it there, d) after selection, treating the clones with a suitable conjugative plasmid and abolishing the mobilizability defect, e) conjugating the clones thus obtained with, preferably plasmid-free, obligate methylotrophic bacteria as recipient and f) selecting the desired clones.