FREMGANGSMAATE VED STABILISERING AV EKSTRA-KROMOSOMALE ELEMENTER I BAKTERIER UNDER DYRKING OG FREMGANGSMAATE FOR FREMSTILLING AV ET OENSKET PRODUKT I TRANSFORMERTE BAKTERIER

A novel method for stabilization of extra-chromosomal elements in bacteria during cultivation without use of antibiotics in the medium has been developed. The method comprises transformation of a host bacterium having a defect in a chromosomal gene needed for the synthesis or maintenance of the cell...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
1. Verfasser:	DIDERICHSEN, BOERGE KRAG
Format:	Patent
Sprache:	nor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A novel method for stabilization of extra-chromosomal elements in bacteria during cultivation without use of antibiotics in the medium has been developed. The method comprises transformation of a host bacterium having a defect in a chromosomal gene needed for the synthesis or maintenance of the cell envelope with an extra-chromosomal element capable of suppressing the requirement caused by the chromosomal gene defect of the host bacterium. The invention furthermore provides for such transformed bacteria and stabilized extra-chromosomal elements and for a method for producing desirable products by inserting a gene for the desirable product into a stabilized extra-chromosomal element and cultivation of suitable host bacteria containing the stabilized extra-chromosomal element comprising the gene for the desirable product. It was demonstrated that according to the principles of this method, a plasmid including the dal gene of Bacillus subtilis and a gene encoding a desirable product can be maintained in an appropriate dalB. subtilis host in conventional media. The method thus allows for improved product yield and quality.