CODOMINANT SSR MARKERS CLOSELY LINKED TO NORNICOTINE GENE qnNIC, AND USE THEREOF

The present disclosure provides codominant SSR markers closely linked to a nornicotine gene qulC and a use thereof. The codominant SSR markers closely linked 5 to the nornicotine gene quIC are numbered TM55416 and TMy85136, respectively, and nucleotide sequences of PCR ampliﬁcation products of the n...

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Hauptverfasser:	KE ZHANG, YUKUI LI, HAOWEI SUN, YUNFANG ZHANG, ZHIJUN TONG, DA ZONG, JIWU ZHANG, DAN CHEN, XIAOWEI ZHANG, JINCHU YANG, HAIYAN LI, JIEYUN CAI, CHUNQIONG WANG, DAN LI, JIE LONG
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TRAPPING VINEGAR WINE
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Zusammenfassung:	The present disclosure provides codominant SSR markers closely linked to a nornicotine gene qulC and a use thereof. The codominant SSR markers closely linked 5 to the nornicotine gene quIC are numbered TM55416 and TMy85136, respectively, and nucleotide sequences of PCR ampliﬁcation products of the nornicotine gene qulC are shown in SEQ ID NO: 1 and SEQ ID NO: 2, and SEQ ID NO: 3 and SEQ ID NO: 4, respectively. The use refers to a use of the codominant SSR markers closely linked to the nornicotine gene qulC in detection of presence of the nornicotine gene qulC in 10 genomic DNA of tobacco. Compared with the methods in the prior art, the codominant SSR markers in the present disclosure have characteristics such as high accuracy, high efﬁciency, high stability, convenience, low cost, and no limitation of detection time. Therefore, the molecular markers can be used in marker-assisted selection (MAS) of the nornicotine gene qulC during tobacco breeding. 15