METHOD FOR PREPARING PROTOPLAST OF CORYDALIS SAXICOLA BUNTING LEAF

The invention discloses a method for preparing a protoplast of a Corydalis SaXicola Bunting leaf, comprising the following steps: 1) cutting a mature Corydalis SaXicola Bunting leaf into filaments, carrying out enzymolysis treatment in an enzymolysis solution, with a mass-volume ratio of the Corydalis SaXicola filaments to the enzymolysis solution of l: 8-12 g/mL, and containing 2%-3% of cellulase R-lO and 0.25%-l% of isolating enzyme R-lO in the enzymolysis solution; 2) filtering the mixed solution after enzymolysis with a sterile filter screen to remove plant tissues and cell clusters Without being completely enzymolyzed, taking a filtrate, centrifuging it at SOC-1,400 r/min for 2-5 minutes, and removing a supernatant to obtain a precipitate; and 3) resuspending the precipitate with a CPW lotion, collecting a cell precipitate after centrifugation to obtain the protoplast of the Corydalis SaXicola Bunting leaf. The protoplast prepared by the inventive method has high yield and high activity.