A METHOD FOR THE EFFICIENT INDUCTION OF POLYPLOIDS OF IRIS LACTEA VAR BY COLCHICINE

The present invention discloses a Hethod for the efficient induction of polyploids of Tris lactea var by colchicine, comprising the following steps: 1) seed germination induction; 2) induction treatment; and 3) cotyledon culture. The present invention induces polyploidy by colchicine immersion treatment of Tris lactea var germinating seeds and cotyledon culture, followed by chromosome ploidy and leaf stomatal identification of the obtained mutagenic seedlings, resulting in 229 polyploid Iris lactea var new germplasm; the method is simple to operate, has a short treatment cycle, a high doubling rate and a low proportion of chimeras, and can be used as a breeding method for Iris lactea var new germplasnl The method, is simple and can. be used, as an important method for breeding new germplasm of Tris lactea var, laying the foundation for polyploid breeding of Tris lactea var.