KIT AND METHOD FOR AMPLIFYING FULL-LENGTH mRNA SEQUENCE OF GYPA, GYPB AND GYPE GENE

The present disclosure provides a kit and a method for amplifying full-length mRNA sequences of GYPA, GYPB, and GYPE genes, and belongs to the field of blood grouping. The present disclosure adopts nested PCR amplification composed of pre-amplif1cation and fragment amplification, which can enhance t...

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Hauptverfasser:	LONG PENG, YUQING SU, FAN WU, SHUANG LIANG, XIONGCHI TANG, JIANWEI REN, YUNPING XU, YANLIAN LIANG, FULING ZHONG, PINGPING SUN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides a kit and a method for amplifying full-length mRNA sequences of GYPA, GYPB, and GYPE genes, and belongs to the field of blood grouping. The present disclosure adopts nested PCR amplification composed of pre-amplif1cation and fragment amplification, which can enhance the specificity and high efficiency of target fragment amplification, so that the full-length mRNA sequences of the GYPA, GYPB, and GYPE genes are obtained.