NOVEL HIGH-OLEIC ACID ALLELIC MUTATION OF BRASSICA NAPUS BnFAD2 GENE AND DEVELOPMENT AND USE OF SNP LABELING PRIMER THEREOF

The present disclosure relates to a novel high-oleic acid allelic mutation of a Brassica napas BnFADZ gene and relates to SNP molecular marker development and a molecular marker assisted 5 breeding technology of a novel high-oleic acid allelic variation. The present disclosure discloses two pairs of novel high-oleic acid allelic mutations (BnAfadZa and BanadZa) of the BnFADZ gene in Brassica napas. The gene has nucleotide sequences shown as SEQ ID N021 and ID NO:2. The present disclosure further provides two pairs of specific KASP molecular markers KASP-421 and KASP-1073 which can rapidly screen novel high-oleic acid allelic mutation sites, and the 10 markers are used for gene identification of a single plant and screening of a high-oleic acid strain. The provided two pairs of KASP molecular markers by the present disclosure are both developed from a SNP variation of an exon region of the Brassica napus BnFAD2 gene and have higher specificity in screening results and more accurate and reliable detection results. The provided KASP molecular markers by the present disclosure only need two steps: PCR and fluorescence 15 detection, have low cost and high throughput, and are particularly suitable for classified screening and identification of high-oleic acid genotypes of offsprings of breeding populations. A combination of the two molecular markers can give offspring materials with the oleic acid content as high as 88% through screening.