DIELECTROPHORETIC MICROFLUIDIC APPARATUS AND METHOD FOR USE IN ISOLATION AND CHARACTERIZATION OF ESKAPE PATHOGENS

In accordance to one aspect, the present invention relates to a dielectrophoretic, microfluidic apparatus (100) for use in detection, characterization, manipulation and isolation of ESKAPE pathogens (200a) disposed in a microfluidic sample of fluid (200). The apparatus (100) comprises at least one p...

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Bibliographische Detailangaben
Hauptverfasser:	Muhammad Ramdzan Buyong, Jacinta Santhanam, Muhammad Khairul Anwar Abdul Rahim, Azrul Azlan Hamzah, Nur Mas Ayu Jamaludin
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PROCESSES OF PREPARING SUCH COMPOSITIONS SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	In accordance to one aspect, the present invention relates to a dielectrophoretic, microfluidic apparatus (100) for use in detection, characterization, manipulation and isolation of ESKAPE pathogens (200a) disposed in a microfluidic sample of fluid (200). The apparatus (100) comprises at least one pair of tapered microelectrodes (105) disposed on a substrate (106), the substrate (106) and the pair of tapered microelectrodes (105) delineating a cavity (107) capable of retention of a microfluidic sample of fluid (200) and accommodating flow of said microfluidic sample of fluid (200); wherein the at least one pair of tapered microelectrodes (105) are connected to an alternating current (A.C) power supply (108) configured to provide A.C electrical power at at a voltage ranging from 5 V (peak to peak) to 10 V (peak to peak) and a frequency at or about any one of the frequencies of 0.62 MHz, 0.69 MHz, 0.76 MHz, 0.91 MHz, 1.07 MHz, and 1.17 MHz which correspond to cross-over frequencies unique to ESKAPE pathogens (200a).