A METHOD FOR QUANTIFICATION OF SIALIC ACID IN EDIBLE BIRD NEST (EBN) WITHOUT DERIVATIZATION PROCESS

The present invention relates to a method for quantification of sialic acid in edible bird nest (EBN) without derivatization process. The method comprises the steps of preparation of standard sialic acid solutions, preparation of Edible Bird Nest (EBN) samples, chromatographic conditions wherein sai...

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Hauptverfasser: SARIFAH BINTI REJAB, AIDAWATI BINTI MOHAMED SHABERY, THEANMALAR MASILAMANI, LIM CHUAN GEE, THAVAMANITHEVI SUBRAMANIAM, DURGAH DEVI
Format: Patent
Sprache:eng
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Zusammenfassung:The present invention relates to a method for quantification of sialic acid in edible bird nest (EBN) without derivatization process. The method comprises the steps of preparation of standard sialic acid solutions, preparation of Edible Bird Nest (EBN) samples, chromatographic conditions wherein said EBN samples undergo hydrolysis process during EBN sample preparation to quantify sialic acid from glycoprotein process without derivatization with a minimum quantity of the EBN sample.