METHOD FOR SIMULTANEOUS BIOSENSING DETECTION OF UREA AND CREATININE

The present invention relates to relates to a Kretschmann-based Surface Plasmon Resonance (SPR) biosensor for simultaneous biosensing detection. The biosensor comprising at least a first channel and a second channel wherein the first channel detects the presence of urea and the second channel detect...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	FAIRUS ATIDA SAID, BURHANUDDIN YEOP MAJLIS, P Susthitha Menon N V Visvanathan
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The present invention relates to relates to a Kretschmann-based Surface Plasmon Resonance (SPR) biosensor for simultaneous biosensing detection. The biosensor comprising at least a first channel and a second channel wherein the first channel detects the presence of urea and the second channel detects the presence of creatine in a sample. The first channel and the second channel are affixed to a nano-laminated gold film with a 50 nm thickness wherein binding between urea and enzyme urease and binding between creatinine and enzyme creatinase in the first and the second channel, respectively, to produce a signal shift in the SPR biosensor using an angular interrogation technique. (Figure 2)