DERMAL FIBROBLAST CONDITIONED SERA BASED ON DEFINED KERATINOCYTE-SPECIFIC MEDIUM FOR SKIN REGENERATION AND REJUVENATION

DERMAL FIBROBLAST CONDITIONED SERA BASED ON DEFINED KERATINOCYTE-SPECIFIC MEDIUM FOR SKIN REGENERATION AND REJUVENATION

This invention relates to a method of producing a medium conditioned using dermal fibroblasts in a defined keratinocyte-specific medium, and a skin rejuvenation composition derived thereof. More particularly, the method of preparing a conditioned cell culture medium, comprising the steps of: culturi...

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Bibliographische Detailangaben
Hauptverfasser: MANIRA BINTI MAAROF, NURUL `IZZAH BINTI HAJI ABDUL GHANI, RUSZYMAH BINTI HAJI IDRUS, SHIPLU ROY CHODHURY
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:This invention relates to a method of producing a medium conditioned using dermal fibroblasts in a defined keratinocyte-specific medium, and a skin rejuvenation composition derived thereof. More particularly, the method of preparing a conditioned cell culture medium, comprising the steps of: culturing dermal fibroblast cells in a culture medium comprising a serum-free keratinocyte-specific medium supplemented with stimulatory factors until the medium contains fibroblast secreted proteins of fibronectin, serum albumin, collagen alpha-1(VI) chain, collagen alpha-1(I) chain, decorin, serotransferrin, pentraxin- related protein PTX3 and metalloproteinase inhibitor 1.