ARTIFICIAL SEEDS OF SAINFOIN

THE PRESENT INVENTION RELATES TO A METHOD OF MICROPROPAGATION OF SAINFOIN. IN THE OPTIONAL FIRST STEP, THE SEEDS OF SAINFOIN ARE STERILIZED. THE BEST STERILIZATION PROCEDURES ARE ACHIEVED WHEN 50% CHLOROX (OUTSIDE THE LAMINAR CHAMBER-1 MIN) AND 70% OF ALCOHOL (INSIDE THE LAMINAR CHAMBER-1 MIN) ARE TREATED ON SEEDS, RESPECTIVELY. THE SEEDS ARE THEN PUT INTO MURASHIGE AND SKOOG MEDIUM (MS) SUPPLEMENTED WITH 3% (W/V) SUCROSE AND 0.75% (W/V) AGAR. AFTER 1-2 WEEKS, ALMOST 100% OF SEEDS ARE GERMINATED. EXPLANT SOURCES, SUCH AS LEAF AND STEM AND ROOT SEGMENTS ARE PREFERABLY DERIVED FROM ASEPTIC SEEDLINGS.