HYDROXYLASES AND MODULATORS THEREOF

A method of identifying, screening, characterising or designing a chemical entity, which mimics or binds to FIH, is described. The method comprises comparing a structural model of FIH with a structural model for said chemical entity, wherein said structural model of FIH is derived from structural factors or structural coordinates determined by subjecting to X-ray diffraction measurements a crystal comprising FIH. Such chemical entities may be used in the treatment of a condition associated with increased or decreased HIF levels or activity. Se describe un metodo para identificar, separar por exclusion, caracterizar o disenar una entidad quimica, que imita o enlaza a FIH. El metodo comprende comparar el modelo estructural de FIH con el modelo estructural de la entidad quimica, en donde el modelo estructural de FIH se deriva de factores estructurales o coordinados estructurales determinados al someter a mediciones de difraccion de rayos X un cristal que comprende FIH. Tales entidades isquemicas pueden usarse en el tratamiento de una condicion asociada con el niveles elevados o reducidos de HIF o actividad.