THERMOSTABLE TAQ POLYMERASE FRAGMENT

It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vecto...

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1. Verfasser:	JESSICA STREY
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS SUGARS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid. Se encontro que un fragmento de ADN polimerasa Thermus aquaticus nativa (TaqWT) que carece de 288 aminoacidos N-terminal (TaqD288), posee una mayor termoestabilidad sobre las TaqWT, TaqD279, y TaqD289. La presente invencion por lo tanto proporciona la TaqD288, vectores de expresion recombinantes que codifican para la misma o derivados de la misma, asi como protocolo de purificacion por TaqD288. La invencion tambien abarca equipos que contienen TaqD288 asi como el uso de la TaqD288 y equipos que contienen TaqD288. Ademas, la invencion abarca metodos para el secuenciamiento de un patron de acido nucleico y metodos para amplificar un acido nucleico objetivo.