THE PRODUCTION OF SOLUBLE KERATIN DERIVATIVES

A process for the preparation of soluble proteins of high molecular weight with little or no damage to the structural integrity of the proteins. The process is economically and environmentally acceptable by virtue of the cost of reagents that are used, and the recycling of some of those reagents, an...

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Bibliographische Detailangaben
1. Verfasser: DIANE JOYCE SINGLETON
Format: Patent
Sprache:eng ; spa
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Zusammenfassung:A process for the preparation of soluble proteins of high molecular weight with little or no damage to the structural integrity of the proteins. The process is economically and environmentally acceptable by virtue of the cost of reagents that are used, and the recycling of some of those reagents, and is suitable for the production of soluble proteins on a large scale. The process includes a first stage using oxidative sulfitolysis followed by a second stage using mild conditions to extract the soluble protein. In the case of wool as the protein source the process leads to the production of soluble keratin proteins fractionated into the classes S-sulfonated keratin intermediate filament proteins and S-sulfontated keratin high sulfur proteins. Se describe un procedimiento para la preparacion de proteinas solubles de alto peso molecular con dano pequeno o sin dano a la integridad estructural de las proteinas. El procedimiento es adecuado economica y ambientalmente en virtud del costo de reactivos que son usados, y el reciclado de algunos de estos agentes y es adecuado para la produccion de proteinas solubles en una gran escala. El procedimiento incluye una primer etapa usando sulfitolisis oxidante seguido por una segunda etapa usando condiciones suaves para extraer la proteina soluble. En el caso de lana como la fuente de proteina el procedimiento lleva a la produccion de las proteinas de queratina soluble fraccionadas a las clases de proteinas de filamento intermedio de queratina S-sulfonada y proteinas de azufre alto de queratina S-sulfonada.